Hi everyone!

we are performing siRNA infusion in mouse brain and, usually it works :-)
but this time we have an unexpected effect: the target gene (protein level) is increased in mice treated with the specific siRNA. It means the protein level is higher in the mice treated with the spe-siRNA than in the control siRNA. this control siRNA was already used by us several times and did not cause any adverse problems before. Does any have an explanation?
I thought it could be interferon response, but how will such response target especially our gene of interest?
thanks for any help

what is your target?

Does this siRNA construct contain the full CDS of your target gene? If so, I am wondering if the cell is somehow translating the RNA that is transcribed from that construct and making protein. If it does not have a full CDS then I have no explanation.