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re-sealing in patch clamp

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asterix
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Topic Started by asterix
on 9/7/2012 9:43 AM   
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We are having problems with our patch electrodes resealing after 2 or 3 minutes. the resistance is great ( 2.5-3), we obtain Gigaseals ina more or less fast, the series resistance is very good (~7) and then the electrode re-seal, and we battle for a while breaking the seal, but it reseal again and finally we kill the cell. Any advice?
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chrismathes
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Posted By chrismathes
on 9/8/2012 7:29 AM   
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 It sounds like you have a pressure leak. Check the o-ring in your holder. 
asterix
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Posted By asterix
on 9/10/2012 6:20 AM   
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