Quantitaive Analysis of Folic acid in Premix .
I have modified IP method :-Modified one
1) System Suitability :-
Mobile phase :- Buffer 93 part and water 7 part
2) Mobile phase :- 0.05 M of KH
2PO
4 and ACN ( 93: 7) ,Adjust
pH 6.0 by using 1 M NaOH sol.
3) Standard solution :- 20 mg dissolve in 100 ml of 0.1 M NaOH ,5 ml of it dissolve in 100 ml of
Mobile phase i.e 10ppm solution .
4)
Sample solution :- Equivalent to 20 mg of folic acid premix dissolve in 100 ml of 0.1 M NaOH ,
centrifuge than 5 ml of supernatant dissolve in 100 ml of mobile phase . i.e 10 ppm solution .
Observed Result : -
Assay 94.56 %, RSD % (n= 5 replicate ) :- Std = 3.40 % and Spl = 3.32 %
Formula :- 1 :- Reference
column (desired one ) ,2 :- Second
column (undesire one )
r :- radius , L :- length F:- Flow rate
(
Column dimension 200 mm x 4.6 mm to desired one 250 mm x 4.6 mm achieve only by Adusting Flow rate )
F
2 = F
1 x L
2 / L
1 x ( r
2/r
1)
2 ; As ( r
2/r
1)
2 = (4.6/4.6)
2 =1
So, 2 = F
1 x 200/250 x 1 ; F
1 = 2.5 ml / min..
I have performed this method , Can i assign it a develop method by giving a RSD limit ?.
"Dr
Analytical sir" please give your comment on this article .
I am Eagerly waiting for your reply.
Thank you !!!!!