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Membrane protein quantification

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hermione
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Topic Started by hermione
on 7/19/2012 12:03 PM   
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Hi friends,

I need help with membrane protein quantification. I already use a  Kit for protein quantification, but membrane proteins are insoluble. So, I am not sure how to quanitify it using the same kit.

I wanna know whether boiling the protein in NaOH might help?

 
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Pippuri
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Posted By Pippuri
on 7/22/2012 5:57 AM   
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Hi hermione,

Two questions:
1) What kind of buffer is your membrane proteins stored in?
2) What kind of kit you are using right now?
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