Hi
I need some data, or a good web page for FRAP.
Could you please assist me

http://www.olympusconfocal.com/applications/literature/frapreferences.html

http://www.olympusfluoview.com/applications/flipandfrap.html

http://www.hopkinsmedicine.org/micfac/frapdemo.ppt

http://www.microscopyu.com/articles/fluorescence/fluorescenceintro.html

http://www.bio.davidson.edu/Courses/Molbio/FRAPx/FRAP.html

http://www-cellbio.med.unc.edu/facilities/frap.htm

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1303450

http://www.answers.com/topic/fluorescence-recovery-after-photobleaching

http://eclipse.nichd.nih.gov/nichd/annualreport/2004/cbmb/sob.htm

http://www.biophysics.org/discussions/2004/kenworthy.pdf

http://www.annalsnyas.org/cgi/content/full/1038/1/115

http://stke.sciencemag.org/cgi/content/full/sigtrans;2005/314/tr28

http://www.bio.davidson.edu/COURSES/Molbio/frap/GFPpb1labob.html

http://www.nature.com/nbt/journal/v22/n11/full/nbt1104-1374.html

http://en.wikipedia.org/wiki/Fluorescence_recovery_after_photobleaching

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=14507689&dopt=Abstract

Million thanks for the above list.

Hello, sorry in addition to all that useful information, does anyone know the largest area you can photo bleach, and if you are using a confocal system, does it photobleach on one specific plane? or am I able to bleach a whole area of a cell?

Depending on your system, you are able to select the area that you want to bleach by drawing on your image with an area marker in the computer imaging program. The area that you bleach is determined by your image size on the screen. It is generally accepted that you will bleach a portion of the area on the screen to analyze the bleached area and the area that is not bleached. Recovery of the bleached area depends on dye transfer from adjacent cells--therefore you want to have adjacent cells that are not bleached