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shani_dr
Israel

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Status: Frog Egg
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Topic Started by shani_dr
on 7/11/2012 3:21 AM   
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 HI all..

I did western today and notice that I saw only  5  (from 6 ) - 50 kd  bands of the marker.

in addition, in the ponceau I saw bands only untill the 50 kd marker.

meaning, I dont see protiens that are smaller than 50kd.

what could be the problem?

Shani
 
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ARGERINE
India

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Posted By ARGERINE
on 7/11/2012 3:33 AM   
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 Hi Shani_Dr

There can be multiple reasons or it:
1) you are using a membrane wit high pore size and transferred it for long duration leading to escape of your LMW proteins

2) A bubble or air space between your gel and membrane

3) Ponceau has alot lower sensitivity in comparison to CBB g250 and as a result youre unable to detect proteins at this range. Increase the loading amount and you may be able to visualize them.

4) degraded proteins in LMW region 

Gaganjot Singh Truth seems so closer now......
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