Dear All,
I'm trying to get the soluble expression of Fab(Fragment of Antibody) in cytoplasma of E.coli.I have gone through the no of litratures and tried some experiment but not getting enough expression.
Actually my question is that ,What we can do as a upstream member (fermentation) to get the good level of soluble expression in e.coli?
I have tried lower temprature,lower agitation,low level of inducer concentration and used some modified media also to get the soluble expression but still not getting enough expression levels.
Please give your suggestions.
Thanks
Dccup43