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Starvartion of Cells

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dennis89
Turkey

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Topic Started by dennis89
on 6/26/2012 12:13 PM   
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 I use MCF-10A cell line (human epithelial cell). I should starvation of cell for cell adhesion. So Which one protocol is available for MCF-10A. Which medium is the best for starvation? E.g: Can I use serum free-medium to starved  MCF-10A cells? Thank you... Please give immediate answers.

Deniz.
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ARGERINE
India

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Posted By ARGERINE
on 6/28/2012 9:31 AM   
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 Hi  Dennis

Starvation is performed in your base medium without serum. The duration of starvation is varaible between 2 h to 48 h. So check accordingly as per required by yr experiment.

All the Best

Gaganjot Singh Truth seems so closer now......
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