Hello! I would like to detect human intracellular IFNb by Western Blot. Does anybody know a protocol or suitable antibody. Also, do you know if it is feasable to detect IFNb from cell lysates by ELISA? Thanks for any suggestions.
A colleague of mine used this Abcam antibody a while back. I don't have a protocol but it apparently worked well. I'll see if I can get the dilutions he used.
It is certainly possible to detect IFNb in lysate.
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