Scientist Solutions: International Life Science Community By Scientists For Scientists
    

Thanks to our sponsors who make this site possible
Search forums by keyword   or   Create a new topic
 Link To Us    Bookmark Us     Chinese

Protein Native gels

 RSS Feed

Would you like to save this topic, event, protocol or job so you can find it again easily?

Just click the "Save to My Lab Drawer" link and the item will be saved in the My Lab Drawer section of your bench space.

Available to members only. Please log in or register for your free account now.

Close Window 
View Printable Version
adlheras
Spain

Send PM
See Mini bio

Status: Frog Egg
Frog Egg
Topic Started by adlheras
on 6/13/2012 3:53 AM   
Reply to this post Go to the top of the page

Hi ,

I trying to run a purified 29 KDa protein in Native gels, but always it is stuck in the wells ( when I' m not using stackig gel) or at the beguining of the separating gel. This protein is a dimer with a pI of 8.3 , i have tried to run it in native gels with pH 9.3 but it doesn' t work, it is still stuck in the wells. Does anybody knows wich could be the problem ?? 

Thank you very much !!
As a Scientist Solutions member, you are able to register a positive vote for any topic which you believe is useful and relevant to our board or any reply which you believe is especially well worded and helpful.

By participating in the voting, you will be helping to identify the best topics & replies on the board.

You may vote once for any one post, and you may not vote for your own posts.

A post (topic or reply) will earn one "thumbs up" icon for every 10 votes received (up to 3 thumbs up), and the person who made the post will also earn two bonus points.

learn more about member points.

Close Window 


Board Rules | advertise | Privacy

Copyright © 2004-2012 Scientist Solutions, All Rights Reserved.
Resources from NCBI used on this site.