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removing erythrocytes from

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nymphaea
Turkey

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Topic Started by nymphaea
on 6/10/2012 12:17 PM   
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Hi everyone!
I'm trying to make primary culture of larynx tumour tissue.  But unfortunately  I can't remove erythrocytes on my flasks. Do you have any idea how to get rid of them?

Thanks
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ARGERINE
India

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Posted By ARGERINE
on 6/12/2012 4:36 AM   
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Hi
You dont have to worry about them at all. After your first media change, RBCs will not be there as they dont adhere to the culture vessel surface.
Give your cells wash with PBS/HBSS/Media and you will have only primary tumor cells only.

Alternatively, you can use the RBC lysis solution from Sigma-Aldrich.

All the Best 

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