I am new to research and was recently given a list of mass spec. data: proteins that are present in conditioned media from mouse cancer cells grown in a monolayer or mouse cancer cells grown in a 3D matrix (there are about a 1000 proteins). I need to start a project to identify if any proteins secreted by mouse cancer cells grown in a 3D matrix inhibit the growth of monolayer mouse cancer cells. Where do I begin??
I have thought of using RNAi to knockdown gene expression of cells in the 3D matrix but I would initially need to knockdown gene expression one at a time. ...and what if inhibition is regulated by a cascade or multiple proteins (which I'm guessing it is), I would have to try all the proteins in a matrix - this seems virtually impossible!!
Is there a different approach that I can try?? A screening process?? Data mining??
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