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Topic Started by ConFiguraTioN
on 2/28/2012 22:50 PM   
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Should I add DMSO for my PCR protocol, the %CG is around 65.
If i do then how much?

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Posted By Ivan
on 2/29/2012 5:18 AM   
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There are many different reagents that can be added to a PCR reaction to get around a high GC content. DMSO is one of them, so is Betaine. One starting point to test DMSO in PCR is 5%. You may want to test other percentages in case 5% does not work.

Ivan Delgado Orlic Carlsbad, CA

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