i am trying to do the comet assay. My aim is to compare the antioxidant capacity.
I use H2O2 for give damage to cells. My problem is I can not see same effect all of the cells which i gave the of damage. Some part of cells look comet while another part is look as if positive control.
In addition when i examining the cells under fluorescent microscope i can see cells in different focus . Does this common problem ? What could be the reasons?
Could you please share your information with me