Hi there,
I'm new to the whole virus studying world and I'm working on a line of cells that are new to me, MDCK cells. Is it true that if they become over confluent they start to transform?
I've been having a hard time finding images of transformed vs non-transformed MDCK cells.
The post-doc in my lab has told me that my cells have changed (and I've thrown away and regrew cells twice already) but they still look similar to when I first seeded them (in my opinion), the only difference I see is that they are confluent. Not that I don't trust the post-doc, but I'd just like a second opinion and if possible if people have images of the differences so I can compare between the two. What does a normal MDCK cell look like vs a transformed one. (what are key features that I should look for to tell the difference?)
I looked online and found some journals and those images looks similar to my cells that the post-doc said was transformed so I'm really confused right now. I probably learn in a different way than they do so it is probably fustrating for them to teach me. Any help would be greatly appreciated! Thanks so much!
I've attached the image from a journal that is MDCK cells and should be normal/not transformed (right?)
Thanks!