SIM Annual Meeting and Exhibition 2006
Pre-Meeting Workshops
Sunday, July 30

http://www.simhq.org/html/meetings.html

All workshops will be held at the Center of Marine Biotechnology, University of Maryland Biotechnology Institute.

REGISTRATION IS OPEN FOR WORKSHOPS AND THE SIM ANNUAL MEETING. Visit the Meetings page of SIM's website for more information.

Price for each day-long workshop
SIM Members: $375
Non-Members: $525
After June 30 add: $50


Cell Culture Techniques (wet workshop)
Conveners: Winona Wagner, Dupont and Julia Cino, New Brunswick Scientific, Co.

This workshop will provide hands-on experience with bench top bioreactors. Participants will prepare an autoclavable bioreactor vessel as configured for a suspension culture run. Vessel configurations for the culture of anchorage dependent cells will be demonstrated. Participants will learn how to calibrate DO and pH probes, observe a running culture and review data captured from the process. Laboratory work will be augmented by presentations and discussions on media development, equipment (sensors, controllers) and methods (control strategies), scale-up and downstream processing.

Faculty and topic:
Probes
pH and dissolved oxygen sensor use and troubleshooting: Practical solutions to common problems
Michael Benning, Hamilton Co., Reno, NV

Downstream Processing
Product recovery from cell culture: Strategies for downstream processing
R.M. Kennedy, GE Healthcare, Piscataway, NJ

Protein Expression: Things to Consider When Expressing a New Protein
Convener: Robert Petrovich, NIH/NIEHS

In this post-genomics era, one of the biggest hurdles is how to express the protein of interests. In this workshop we will discuss some approaches to the expression of a new target protein. The discussion will cover our experiences with: different affinity/solubility tags, how media composition and growth conditions can effect solubility/expression levels, what to consider when choosing a protease to remove the tag and some of the problems encountered in scale up refolding. The participants should gain a basic understanding of some of the tools available when considering the expression of a new protein.

Faculty and topic:
Fermentation optimization, scale up and technology transfer: Tales from the frontline in contract manufacturing
E. Timothy Davies, University of Georgia, Athens, GA

Optimization of soluble protein expression
Dominic Esposito, SAIC Frederick

Refolding difficult and challenging proteins
Lyndal Hesterberg, BaroFold, Inc.

Protein expression: Is there one "Best" way to do it?
Robert Petrovich

Protein production by auto-induction in inducible T7 expression systems
F. William Sudier, Brookhaven National Laboratory

A one-size-fits-most strategy for the production of recombinant proteins in Escherichia coli
David S. Waugh, National Cancer Institute, NIH


Design and Optimization of Growth Medium
Convener: Randy Greasham, Consultant

Medium design and optimization are key components in the development of a highly productive, consistent and scaleable fermentation process. Rapid process development requires these components to be developed in a time and resource efficient manner. This workshop shall focus on the physiological logic behind defining the nutritional components of growth medium, the control of the growth and production phases through process engineering, and their efficient optimization. A primary focus of the course will be a practical overview of the use of Statistical Design of Experiment in the optimization of growth medium and process conditions. The content of this workshop shall apply to all fermentations from bacteria to cell culture. Those attending should gain sufficient practical knowledge, literature, and supporting materials to implement a media design and optimization program.

Faculty and topic:
Randy Greasham, Consultant
Max Kennedy, IRL-Biopharma


For more information on workshop schedules, registration and confirmed sessions at SIM's Annual Meeting and Exhibition 2006, please visit SIM's MEETINGS PAGE today!