| SRep said: |
I've recently been working on designing chimeras of mouse GPCRs for electrophys. experiments. The "parent" receptors express well, and I believe that my alignment of them was correct. I cannot seem to get any chimeras to work-- I've done some reading, and there is conflicting evidence as to where I should design the "joints" for the chimeras.
Does anyone have any experience with this?? Any help would be greatly appreciated- my dissertation is on standstill until i can figure this out |
Your problem is not very clear.
However, if your problem is the construction of chimera between two GPCRs some reading may be of help:
Dezhong Yin, Shai Gavi, Hsien-yu Wang, and Craig C. Malbon Probing Receptor Structure/Function with Chimeric G-Protein-Coupled Receptors Mol Pharmacol 65:1323-1332, 2004.
In this minirev you'll find a table summarizing the huge work that have been published regarding the studies of receptor structure/function by construction of chimeric GPCRs.
GOOD LUCK.
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