I have some question about angiogenesis method. I have a problem in experiment.
I do Spheroid assay
for 3D spheroid by Hanging drop technique
. Using matrix by collagen
tails so my matrix is not clear I think it participate of collagen
, because it has a white fibrous whan see in well and debris in microscope
From this problem impact for spheroid is not good,not circle, cell
of this expose form there in next time so don't see abnormal shape in first day. I think this problem happens form collagen
hasn't well of polymerization.
How can do it ? I do collagen
on ice 3-5 minitue.