ubiquitin-proteasome pathway [View Printable]
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omid
Group: Member
Posts: 59
Joined: Dec 16, 2004
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Ubiquitination is the targeting of proteins for degradation by the 26S proteasome. Since it is a reversible post-translational modification, I am looking for best methods or protocols designed to prevent, reverse or at least to monitor the ubiquitination process. Anybody can help? |
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| Posted Dec 17, 2004, 1:05 AM |
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DD
Group: Member
Posts: 64
Joined: Dec 17, 2004
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| omid said: | | Ubiquitination is the targeting of proteins for degradation by the 26S proteasome. Since it is a reversible post-translational modification, I am looking for best methods or protocols designed to prevent, reverse or at least to monitor the ubiquitination process. Anybody can help? |
Omid, Proteasome is an enzyme complex within the cell responsible for breaking down proteins marked for removal by the attachment of ubiquitin. Inhibition of the proteasome prevents the targeted proteolysis by ubiquitin to occur. Check the company called: Millennium Pharmaceuticals. They are developping and launching into the cloinical trial a proteasome inhibitor called: VELCADE. Call: 1-866-835-2233 or 1-800-589-9005 |
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| Posted Dec 19, 2004, 19:02 PM |
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darsau
Group: Member
Posts: 4
Joined: Dec 21, 2004
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| Posted Jan 13, 2005, 17:12 PM |
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samm
Group: Moderators
Posts: 407
Joined: Mar 03, 2005
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Look up the Calbiochem catalog - they have a specific heading called proteasome inhibitors that covers both direct inhibitors and inhibitors of ubiquitin ligases, and related enzymes. Among the dirct inhibitors, i have successfully used a combination of ALLN and MG132. |
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| Posted Mar 14, 2005, 20:37 PM |
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DD
Group: Member
Posts: 64
Joined: Dec 17, 2004
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Research into proteasome fubctions in cancer has been stimulated since the discovery and US food and Drug Administration approval of "Velcade" a proteasome inhibitor and promissing treatment for multiple myeloma. Yet it would be premature to say that cancer itself is a disease of protein homeostasis. Any ideas or opinions? |
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| Posted May 29, 2005, 4:44 AM |
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