Hi

I have a problem when runing by protein sample in SDS-PAGE. The problem occures as i turn on the power and the samples start to move down since all wells or the dye in the bands get conceted or merged for no clear reson. I though this can be a result of incomplete polymerization of wells, so i kept it for a longer time but still the problem occurs. Moreover my sample volume is not too large, it is only 20 ul which excludes the possibility of over flowing. This conncetion of bands is causing the bands after bloting and developing to be unclear exhibiting the connected patern. such merging distort the result. Can any one help on this please.

Thanks

Zeina

Hi
I will try to help,
More data is needed for that.
What is the gel %,
Do you do seperating and staking
Do you use Constant Voltage if so how many Volts do you use?
Guy

Zeina,

I have seen this problem when there is too much salt (NaCl, urea, etc) in my samples. If suggestions for pouring the gel dont pan out, try to desalt your samples by by simple chromatoraphy methods like the Zeba Desalt column from Pierce or some similar device.

RB