| Sandy said: |
Can someone describe the analytical strategies for "shotgun proteomics" and provide name of companies selling the technology and or related equipments ?
Thanks for your help |
The LC-MS/MS procedure of shotgun proteomics is known an "unbiased" approach because it is not biased as to the identity or number of proteins that could be detected in a single sample. But, as Dr. DOwd indicated, it can be quite expensive.
Another approach to "shotgun proteomics" is the so-called "biased" approach. These techniques usually employ antigen-specific antibodies in an array format. Although the number and identity of the proteins that can be detected are limited, the sensitivity is quite good...low pg/ml levels in many instances, and the price is very reasonable...the cost of a few antibodies or less.
This is particularly true for investigations involving secreted cell-cell communication via cytokines, chemokines, angiogenic factors, growth and differentiation factors, MMPs and inhibitors and/or soluble receptors of these molecules. There are a number of antibody array products available that can detect these molecules, many with no more specialized equipment than needed to do Western.