|Can someone describe the analytical strategies for "shotgun proteomics" and provide name of companies selling the technology and or related equipments ?|
Thanks for your help
procedure of shotgun proteomics
is known an "unbiased" approach because it is not biased as to the identity or number of proteins
that could be detected in a single sample
. But, as Dr. DOwd indicated, it can be quite expensive.
Another approach to "shotgun proteomics
" is the so-called "biased" approach. These techniques usually employ antigen
in an array
format. Although the number and identity of the proteins
that can be detected are limited, the sensitivity is quite good...low pg/ml levels in many instances, and the price is very reasonable...the cost of a few antibodies
This is particularly true for investigations involving secreted cell
communication via cytokines
, chemokines, angiogenic factors, growth and differentiation factors, MMPs and inhibitors
and/or soluble receptors of these molecules. There are a number of antibody array
products available that can detect these molecules, many with no more specialized equipment than needed to do Western.