Hi evrybody,
Lately I started doing some assays on 12 well plates.
I need to know what is the protein concentration in each well.
Does any one have a good method for that?
Guy

I'm not sure how your assay is set up (are you working with lysates, etc), but I would probably just take an aliquot from each well, stick in on a 96 well plate and run a Bradford assay. For instance, here's a link to a coomassie reagent you could use: http://www.piercenet.com/products/browse.cfm?fldID=02020104

I do Tritiated GABA uptake assays on twelve well plates plated with 300000 transfected N2a cells.

At the end of the assay I lyse my cells in 1ml of 2% SDS in PBS and take a 50ul aliquot of each well to run in 96 well BCA protein assays (Pierce).

This is enough to run up to ten replicates from the same sample in the 96 well format.

Thank you for the answer.
I did it with RIPA buffer for lysis and used BioRad BCA assay.
It works
Guy

Another big Q?
Can I do it after I fixed the cells with 4% Formaldehyde?
Guy