Hello Everyone,

I am looking for a suggestion for a software that performs quantitative colocalization analysis of confocal immunofluorescence images. Is there any of this kind?

Thanks in advance!

Taro

Have you tried Image J and any of its many plug ins?
Freeware at

http://rsb.info.nih.gov/ij/

plug ins:

http://rsb.info.nih.gov/ij/plugins/colocalization.html
http://rsb.info.nih.gov/ij/plugins/colocalization-finder.html
http://rsb.info.nih.gov/ij/plugins/rg2bcolocalization.html

Thanks, I tried Image J and these plug-ins. All of them require to open 2 images to analyze. I always save a single merged image, so this is not suitable to me. What I am looking for is the program that lets me open a merged image and perform calculations.

Still very much appreciate other related information.

There is a software called CoLocalizer Pro. It is Mac-only. It does quantitative colocalization analysis and is very user-friendly. It maybe what you need:

http://homepage.mac.com/colocalizerpro/

ImagePro was recommended to me for colocalization quantification, although I never actually ended up using it. Here's the website of the software maker: http://www.mediacy.com/ THey do say they need separate images. I'm curious as to why you only save hte merge - it would seem to me that you're losing information if you don't save all the images you acquire.

Hi,
I useed ImagePro for several years for everal applications.
It is a good software.

[quote=macbride]ImagePro was recommended to me for colocalization quantification, although I never actually ended up using it. Here's the website of the software maker: http://www.mediacy.com/ THey do say they need separate images. I'm curious as to why you only save hte merge - it would seem to me that you're losing information if you don't save all the images you acquire.

Any quantitative colocalization analysis software takes into consideration 2 images, I think. The only difference is that some programs may need to have 2 of them open, others separate merged images into 2 channels before performing coefficients calculations, users just do not see that.

hey,
hmmm, strange!! actually I don't understand your problem, bcz imageJ split a merged image automatically in 2 or 3 separate images after opening the file. Have you tried this button: Image-->color--> RGB split. and when it does not work, then may be you have to check the format of your file you saved your images in. I am afraid that ImageJ is not able to handle with images which have been saved as a picture.

when i was doing coloc. analysis, i was looking at pretty distinct structures, so i actually just counted them by eye. that was pretty easy and non-arbitrary.