So I just ran my transfer, and my ladder transferred all well and great, BUT when I stained with Ponceau S, I do not see any bands at all! So my question is, should I still proceed and do my primary, secondary etc? Perhaps the Ponceau is not specific enough? Have any of you ever been in this situation, and ran your western and it worked anyways?
Please let me know. Any help would be awesome and you shall be blessed by a good luck midget =)
No but seriously, help! Thank you!