IMMUNOFLUORESCENCE

Hi Everyone,
Could you please suggest me that which type of microplate should I use while doing Immunofluorescence microscopic assay for obtaining a quality image....

Dear Anjielie

I hope the question is regarding the use of coated or non coated plates. In strict terms the choice depends upon the cell type one is working with. If you are working with a non adherent cell line or primary culture It is advisable to use the coated plates or pre coat them using gelatin or Poly lysine. Else If you are working with primary cells that adhere well or adherent cell lines, you may even proceed with regualr cell culture plates.
Regarding number of wells you may proceed with cells and seed them accordingly.

All the best.

Dear Argerine,

Of Course your ans is right  but ..........I think I need to be more specific.........My Query was regarding the colour of microplates to be used...........like for fluorescence assays we normally use black colored plates............similarly for IF should I need to use the same plates with the difference that the plates should be bottom clear for optical view..........this is jst my doubt.........I dnt knw how others r doin IF to get good clarity images.......plz suggest me

Yes black microplates with transparent bases are preferred for fluorescence and luminescence studies to increase the signal to noise ration in each well.

White plates increase the signal but also the background.

the usual order of preference for fluorescence studies is Black>clear>white

Hi TheFFM,

Thank you for the suggestion.