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 Gq-gpcr on neutrophil OR macrophage [View Printable]
odatom

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Please tell me the Gq-coupled GPCRs on neutrophil OR macrophage.
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 Posted Jan 20, 2006, 23:10 PM
montgomj

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Macrophages appear to have M1, M2 and M3 muscrinic acetylcholine receptors (mAChRs). It seems that the M1 and M3 can be Gq-coupled. M2 is Gi/o.

See below for References

1) Muscarinic receptors participation in angiogenic response induced by macrophages from mammary adenocarcinoma-bearing mice
de la Torre E, Davel L, Jasnis MA, Gotoh T, de Lustig ES, Sales ME.
Breast Cancer Res. 2005;7(3):R345-52. Epub 2005 Mar 4.

http://breast-cancer-research.com/content/7/3/R345


2) G protein betagamma11 complex translocation is induced by Gi, Gq and Gs coupling receptors and is regulated by the alpha subunit type.
Cell Signal. 2005 Oct 18; [Epub ahead of print]
Azpiazu I, Akgoz M, Kalyanaraman V, Gautam N.
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T2M-4HC6KXB-1&_coverDate=10%2F19%2F2005&_alid=358792171&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=4922&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=d9fa4081f493299232b4f1721b76a2a9

3) Phospholipid metabolism is required for M1 muscarinic inhibition of N-type calcium current in sympathetic neurons.
Liu L, Roberts ML, Rittenhouse AR.
Eur Biophys J. 2004 May;33(3):255-64. Epub 2004 Mar 5.
http://springerlink.metapress.com/(vrhtz2qi4oj3chrsc0suru55)/app/home/contribution.asp?referrer=parent&backto=issue,12,16;journal,17,188;linkingpublicationresults,1:100412,1

4)Constitutively active Gq/11-coupled receptors enable signaling by co-expressed G(i/o)-coupled receptors.
Bakker RA, Casarosa P, Timmerman H, Smit MJ, Leurs R.
J Biol Chem. 2004 Feb 13;279(7):5152-61. Epub 2003 Nov 10.
http://www.jbc.org/cgi/content/full/279/7/5152
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Histamine H1 receptors are Gq coupled and expressed on neutrophils

1) Pro-apoptotic effect of high concentrations of histamine on human neutrophils.
Hur J, Kang MK, Park JY, Lee SY, Bae YS, Lee SH, Park YM, Kwak JY.
Int Immunopharmacol. 2003 Oct;3(10-11):1491-502.

2) C. Gespach and J.P. Abita, Human polymorphonuclear neutrophils. Pharmacological characterization of histamine receptors mediating the elevation of cAMP. Mol. Pharmacol. 21 (1982), pp. 7885.

3) S. Westcott and M. Kaliner, Histamine H-1 binding site on human polymorphonuclear leukocytes. Inflammation 7 (1983), pp. 291300.
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Posted Jan 26, 2006, 2:56 AM
val

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To my knowledge they express Gq-coupled receptors for leukotriene B4 (BLTR), leukotriene D4 (CysLTR), platelet-activating factor (PAF), interleukin-8 (IL-8), and C5a. Also some kind of purinergic receptors (P2Y2, 4 and 6 in monocyte/macrophage like U937 cells for example).

I don't know the rationale of your research, but be careful to the fact that Gq coupled receptors are generally promiscuous, being coupled also to Gi.
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Posted Jan 31, 2006, 11:29 AM
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