Which companies are proficient in generating trangenic/knockout mice at reasonable prices?

DaJo said:

Which companies are proficient in generating trangenic/knockout mice at reasonable prices?

Numerous Universities now have facilities for the production of transgenic animals and knockouts. I had some generated at UCSD http://cancer.ucsd.edu/Research/Shared/tgm/default2.asp
I was both pleased with the price and the results.

DaJo said:

Which companies are proficient in generating trangenic/knockout mice at reasonable prices?

We've had several knockout/transgenic lines made by Ozgene.

What are transgenics/Knockout animials? What are they typicaly used for?

Sorry, I just love science and this site is a source of learning for me.

Thank you
D

DeeDee said:

What are transgenics/Knockout animials? What are they typicaly used for?

Here is a website giving an introduction to knockout mice:

http://www.wellcome.ac.uk/en/genome/technologies/hg17b005.html

Thank You for that site it was very intresting
D

what is the difference between the Knockout animials and RNAi ?

Knockout mice contain the same, artificially introduced mutation in the gene of interest in every cell, abolishing the activity of a preselected gene.

RNAi (RNA interference) refers to the introduction of homologous double stranded RNA (dsRNA) to specifically target a gene's product, resulting in null or hypomorphic phenotypes. The difference is that the introduction of RNAi into a cell, targets the messenger RNA for the protein of interest, rather than mutating the gene to shut down its expression.

Here is a company that can make custom-made gene overexpression, humanized and Knock-down (RNAi) mice models in 2 months. We haven't used them but I thought I would pass on the info.

http://www.nucleis.com/

Taconic is a great source for mice! Go to www.taconic.com or call 888-taconic to speak to customer service.