You've not given any clues as to why your challenge is anything out of the ordinary. Why are you mentioning to a immunoradiometric assay (IRMA)? Do you have a particular problem with sensitivity? A particular problem with protein binding to your wells?
How big is your peptide?
Is your antibody working?
What are you trying to achieve with your assay - if you've already purified your protein and are trying to quantify the protein there may be better approaches. Or are you planning to use your purified protein to calibrate an ELISA?
Members of the forum will be able to help if best if you ask a specific question.