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 fluorescence in inverted microscopy [View Printable]
Abe

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I am looking for an inverted scope that will allow me to image fluorescently labeled cells on the surface of an opaque material. I am considering the Nikon TS100, but I was also wondering if anyone has used fluorescence with the Olympus CK40 scope.

Any references using this technique would be appreciated.
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 Posted Jan 11, 2006, 20:13 PM
klepikhov

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I am using Zeiss Axiovert 200M both for micromanipulations and Fluorescent observations. Works fine, except of few bugs in the sofware provided. If equipped with ApoTome module - allows to get pictures as good as those from confocal imaging.
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Posted Jan 19, 2006, 10:05 AM
gsovak

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I used to use Zeiss also.
It was a AxioVert200 I was very happy happy with it.
http://www.zeiss.com/4125681F004CA025/?Open
I used the Axiovision softwear which was great.
Guy
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Posted Jan 19, 2006, 14:06 PM
samm

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We currently use a Olympus IX81 frame with the DSU attached. It works great for imaging on Transwell filters - which are largely opaque. Again, the DSU allows z-axis segments - not as fine as LSM, but with no bleaching and a tenth of the price.
We have Slidebook and imagePro software to go with it - controls both microscope functions and image acquistion.
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Posted Jan 19, 2006, 18:22 PM
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