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Lysis bufferes [View Printable]
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gsovak
Group: Member
Posts: 544
Joined: Jan 25, 2005
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Hi all,
I am looking for a good mild lysis buffer.
Would like to get some and try them.
I tryed several but I still want to try nd improve it.
I am using150mM NaCl, 20mM Tris-HCl and 0.1% NP40 pH7.4.
Guy
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| Posted Jan 09, 2006, 11:12 AM |
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gsovak
Group: Member
Posts: 544
Joined: Jan 25, 2005
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Found the solution :-)
My first problem was stricks in my western.
I changed the incubation perod from 20min to 15 min. on Ice.
Guy
Hope it will help some one
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| Posted Jan 19, 2006, 14:29 PM |
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guy
Group: Moderators
Posts: 275
Joined: Nov 28, 2005
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What concentration of protease inhibitors are you using?
Do you use PMSF?
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| Posted Jan 20, 2006, 19:05 PM |
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