Hi to evrybody,
I suspect that my siRNA 3T3 cells has got problems with the plasma membrane.
I thought of checking the cholesterol and looking for a staining method to view the cholesterol.
Someone has got an Idea how to do it.
Thanks
Guy

gsovak said:

Hi to evrybody, I suspect that my siRNA 3T3 cells has got problems with the plasma membrane. I thought of checking the cholesterol and looking for a staining method to view the cholesterol. Someone has got an Idea how to do it. Thanks Guy

Please check this paper:
http://www.molbiolcell.org/cgi/content/full/8/11/2111
with the title:
Cholesterol independent targeting of Golgi membrane proteins in insect cells.

Thenk you
Welcome to our forum
I will check the paper.
Guy