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Identification of mouse peritoneal macrophages

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Xinyu Fee
China

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Topic Started by Xinyu Fee
on 11/1/2010 3:02 AM   
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In my experiments, I need to isolate mouse peritoneal macrophages and identify them, are there any simple approaches to identify them?
Thank you a ton !
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heehawmcduff
United States

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Posted By heehawmcduff
on 11/1/2010 12:25 PM   
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Hi,

The easiest way to identify the macrophages is probably via immunofluorescent staining and flow cytometry.  Many people have used antibodies directed towards the cell surface markers F4/80 and CD11b, but you can also include an intracellular stain for CD68. 

This protocol covers isolation and identification of mouse peritoneal macrophages quite comprehensively.

http://www.ncbi.nlm.nih.gov/pubmed/19016445

Hope that helped.
Denjo
Italy

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Posted By Denjo
on 11/10/2010 6:17 AM   
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Hi,
and if I wished to isolate mouse peritoneal monocytes, aiming to colture them afterward, is the same procedure as for macrophages ok?
heehawmcduff
United States

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Posted By heehawmcduff
on 11/11/2010 18:02 PM   
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Hi

Do you mean the same procedure in the mouse model or the identification post isolation?
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