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Biotinylation and MS [View Printable]
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Harky
Group: Member
Posts: 3
Joined: Dec 08, 2005
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We are currently looking to identify a biotinylated protein by MS. The biotin we used cross-links with the side chains of Lysine residues.
Nobody I have spoken to seems to know whether biotinylation of Lysines will affect the subsequent trypsinization (which cleaves after lysine residues) of the protein. If it does, then the fingerprint we will obtain will be difficult to interpret, if not, then it should be okay.
Anybody have any experience with MS and biotinylated proteins?
Harky
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| Posted Dec 08, 2005, 10:54 AM |
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guy
Group: Moderators
Posts: 291
Joined: Nov 28, 2005
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Hello Harky,
Welcome to our group.
I found an article which maybe can give you an unswer.
www.lumicyte.com/Information/ 2005%20ASMS%20Poster%20TP04-070.pdf
Tell me if it helped.
Guy
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| Posted Dec 08, 2005, 14:46 PM |
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Harky
Group: Member
Posts: 3
Joined: Dec 08, 2005
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Thanks Guy,
Its not exactly what I was looking for but I have emailed the group that performed this work as I have a feeling that they will have a specific answer to my question.
All the best.
Harky
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| Posted Dec 09, 2005, 11:33 AM |
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