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sterility while immunostainings

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soumee
Germany


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Topic Started by soumee
on 9/24/2010 2:15 AM   
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i dnt have too much experience with immunostainings. i generally fix my cells and use them within a weeks time. but i want to know if i should do all the staining steps in sterile conditions because under the microscope i sometimes feel i can see some contamination.


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pundir
India

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Posted By pundir
on 9/24/2010 18:38 PM   
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 Hi soumee,
after you fix the cells there are no chances of any such contamination like bacterial or fungal but if you are keeping the slide or well plates after fixation for a week in any buffer without any anti bacterial or fungal agent(like sod.azide etc or antifreeze etc.) there are chances of contamination so its better to use  any such agent or avoid the delay in doing IHC after fixation..................

impossible! let's do it................



soumee
Germany


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Posted By soumee
on 9/24/2010 6:06 AM   
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thanks pundir



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