Scientist Solutions: International Life Science Community By Scientists For Scientists
    
Home » Forums » Antibody Based Technologies » Assay Development & Protocols » recombinant monoclonal antibody

Thanks to our sponsors who make this site possible
Search forums by keyword   or   Create a new topic
 Link To Us    Bookmark Us     Chinese

recombinant monoclonal antibody

 RSS Feed

Would you like to save this topic, event, protocol or job so you can find it again easily?

Just click the "Save to My Lab Drawer" link and the item will be saved in the My Lab Drawer section of your bench space.

Available to members only. Please log in or register for your free account now.

Close Window 
View Printable Version
wisdom
India

Send PM
See Mini bio

Status: Frog Egg
Frog Egg
Topic Started by wisdom
on 9/9/2010 0:38 AM   
Reply to this post Go to the top of the page

Analytical methods to quantitate and assess charge variants in recombinant monoclonal antibodies and their structural characterisation.
Replies
wisdom
India

Send PM
See Mini bio

Status: Frog Egg
Frog Egg
Posted By wisdom
on 9/9/2010 0:40 AM   
Reply to this post Go to the top of the page

Mol weight of Mab is 150KDa. But on a 10% SDS Non-reducing PAGE it aligns above the 200KDa marker. But after reduction has two bands of approximately 50KDa and 25KDa. What could be the reason?
samm
United States

Send PM
See Mini bio

Status: Cell & Tissue Culture, Antibody Technologies Moderator
Frog Laureate
Posted By samm
on 9/9/2010 10:27 AM   
Reply to this post Go to the top of the page

Glycosylation and marker variability are magnified in non-reducing native conditions - a more stringent technique will show ~155-165 kD for IgG isotypes.
2x55kD+2x25kD in reducing SDS gels.
As a Scientist Solutions member, you are able to register a positive vote for any topic which you believe is useful and relevant to our board or any reply which you believe is especially well worded and helpful.

By participating in the voting, you will be helping to identify the best topics & replies on the board.

You may vote once for any one post, and you may not vote for your own posts.

A post (topic or reply) will earn one "thumbs up" icon for every 10 votes received (up to 3 thumbs up), and the person who made the post will also earn two bonus points.

learn more about member points.

Close Window 


Board Rules | advertise | Privacy

Copyright © 2004-2012 Scientist Solutions, All Rights Reserved.
Resources from NCBI used on this site.