As an unswer to posting from few months ago.
I found a method for destaining Silver stained gels.

Silver destaining protocol

Destaining Solution:
0.4g potassium ferricyanide (K3Fe(CN)6) in 200ml sodium thiosulphate (0.2g/L Na2S2O35H2O, same solution used in silver staining protocol).


Destain:
Incubate gel or gel pieces in destaining solution with gentle shaking until no bands are visible, approximately 15 minutes. The gel will be yellow.
Wash gel 4-5 times for 15 min with plenty of milli-Q H2O until gel is transparent and has no background color.

If you are doing this because no staining was visible first time around, the gel is then ready to be restained with silver starting from the sensitization step, or if you are doing this to get rid of stain, the bands are then ready for in-gel digestion.


Guy

hello
I tried another method for silver distaining. It's good but I wasn't sure whether it's good protocol.

Potassium ferricyanide [K3Fe(CN)6] 1%:solution A
Sodium thiosulfate 1,6%:solution B

100l of solution A + 100l of B to recover spots. Shake gently or ~5 min at room temperature
Wash with ultrapure water sevaral times (~4 times)

Always use 1A:1B
for silver staining, A can be diluted 10 times
more A diluted, more time needed for stianing but less time for washing

What do you think about that?

Giang

Thats a good protocol.
You can also shake for 20min at 4deg.
Guy

i use non ammoniacal silver staining method. once my gel got stained more and it became dark in colour. is there any way i can desain it and restain it without loss of any protein bands.

thanks in anticipation