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EAATs antibodies NEED HELP!

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Topic Started by luciotremolizzo
on 11/16/2005 14:11 PM   
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I have tried to work out the western blotting assays for EAAT-1, -2 and -3 using Chemicon Abs but they did not work very well... now it even seems to me that any previously published gel is not REALLY nice.
Is actually somebody able to get a nice immunoreactivity for EAATs?
Is there any specific problem that I must address?
Thanks.


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frasermoss
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Posted By frasermoss
on 11/17/2005 18:57 PM   
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Are you staining for Mouse, Rat or human EAATs?

Have you asked Jeff D Rothstein for samples of any of his antibodies (Department of Neurology, Johns Hopkins University, Baltimore, MD 21287, USA)?

Search Pubmed for Rothstein JD to get many papers with EAAT immunoprobing.




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Posted By luciotremolizzo
on 11/18/2005 7:03 AM   
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human... I tried the Rothstein Ab previously but I was wondering if there are any commercially available ab that works ok.
thanks in any case

frasermoss said:
Are you staining for Mouse, Rat or human EAATs?

Have you asked Jeff D Rothstein for samples of any of his antibodies (Department of Neurology, Johns Hopkins University, Baltimore, MD 21287, USA)?

Search Pubmed for Rothstein JD to get many papers with EAAT immunoprobing.



frasermoss
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Posted By frasermoss
on 11/18/2005 11:47 AM   
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I cannot vouch for its quality, but I found this Mouse Anti-Human Excitatory Amino Acid Transporter 2 Ab-1 Monoclonal Antibody, Unconjugated, Clone 1H8 from Lab Vision

http://www.labvision.com/ab.cfm?first=AntiBody&second=1750

Search biocompare.com for antibody suppliers



taurine
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Posted By taurine
on 1/1/2006 3:06 AM   
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Suggest look at the papers by Pow DV who has some nice antibodies to all the EAATs including splice variants. There is a company (immunosolution.com) that sells some of them (I think)



taurine
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Posted By taurine
on 1/1/2006 3:19 AM   
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PS see the EAATs website at www.eaats.org as it has some nice images of EAATs immunocytochemistry including human.




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Posted By luciotremolizzo
on 1/1/2006 5:28 AM   
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Thanks a lot... I did not try these Abs before... I'll let you know if I buy them. L



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