EAATs antibodies NEED HELP! |
|
Would you like to save this topic, event, protocol or job so you can find it again easily?
Just click the "Save to My Lab Drawer" link and the item will be saved in the My Lab Drawer section of your bench space.
Available to members only. Please log in or register for your free account now.
|
|
I have tried to work out the western blotting assays for EAAT-1, -2 and -3 using Chemicon Abs but they did not work very well... now it even seems to me that any previously published gel is not REALLY nice. Is actually somebody able to get a nice immunoreactivity for EAATs? Is there any specific problem that I must address? Thanks.
|
Replies
|
|
|
Are you staining for Mouse, Rat or human EAATs?
Have you asked Jeff D Rothstein for samples of any of his antibodies (Department of Neurology, Johns Hopkins University, Baltimore, MD 21287, USA)?
Search Pubmed for Rothstein JD to get many papers with EAAT immunoprobing.
|
|
|
|
human... I tried the Rothstein Ab previously but I was wondering if there are any commercially available ab that works ok. thanks in any case
| frasermoss said: | Are you staining for Mouse, Rat or human EAATs?
Have you asked Jeff D Rothstein for samples of any of his antibodies (Department of Neurology, Johns Hopkins University, Baltimore, MD 21287, USA)?
Search Pubmed for Rothstein JD to get many papers with EAAT immunoprobing.
|
|
|
|
|
|
Posted By taurine
on 1/1/2006 3:06 AM
|
|
Suggest look at the papers by Pow DV who has some nice antibodies to all the EAATs including splice variants. There is a company (immunosolution.com) that sells some of them (I think)
|
|
|
|
Posted By taurine
on 1/1/2006 3:19 AM
|
|
PS see the EAATs website at www.eaats.org as it has some nice images of EAATs immunocytochemistry including human.
|
|
|
|
Thanks a lot... I did not try these Abs before... I'll let you know if I buy them. L
|
|
As a Scientist Solutions member, you are able to register a positive vote for any topic which you believe is useful and relevant to our board or any reply which you believe is especially well worded and helpful.
By participating in the voting, you will be helping to identify the best topics & replies on the board.
You may vote once for any one post, and you may not vote for your own posts.
A post (topic or reply) will earn one "thumbs up" icon for every 10 votes received (up to 3 thumbs up), and the person who made the post will also earn two bonus points.
learn more about member points.
|
Become a member & join our community (It's easy & free)
Already a member? Please log in
|
AbD Serotec
|
|
Biocision
|
|
Gene-Tools
|
|
7/2/2010
|
|
7/2/2010
|
|
7/2/2010
|
|
9/2/2010
|
|
9/2/2010
|
|
9/2/2010
|
|
|