I work with apoptotic mechanisms in neurodegenerative disorders and would like start a discussion regarding new molecules that have been found to be involved in the process of neuronal apoptosis in the past few years. This is regarding molecules that are besides the traditional ones we already know. We could form a data base here for others to dip into. Ofcourse if some information is proprietary its understood. Sometimes molecules are discovered in fields so varying from our own field its difficult to do a lit search all the time......

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parkinsonbuster said:

I work with apoptotic mechanisms in neurodegenerative disorders and would like start a discussion regarding new molecules that have been found to be involved in the process of neuronal apoptosis in the past few years. This is regarding molecules that are besides the traditional ones we already know. We could form a data base here for others to dip into. Ofcourse if some information is proprietary its understood. Sometimes molecules are discovered in fields so varying from our own field its difficult to do a lit search all the time...... FORMAT: MOLECULE: CITATION (if any): SHORT DESCRIPTION:

Could you provide a list of traditional molecules that you already know? A molecule such as Par-4 is relatively new, what about Tau proteins?. Are you referring to these proteins as traditional molecules?

Sounds like a great idea! I am wondering if you are referring to the molecules associated with the cell death pathways through the cytosol and the mitochondria?? So many steps have been identified in these pathways. For example, I can't keep up to all the caspases and intermediate steps. Of course many of these would be applicable to all types of cells outside of neurons.
In relation to this, I am always looking for the best (reliable) method for showing apoptosis via immunocytochemistry in culture. There are so many kits. But is there one method that really stands out ie. works well, stains well etc.

Doering said:

Sounds like a great idea! I am wondering if you are referring to the molecules associated with the cell death pathways through the cytosol and the mitochondria?? So many steps have been identified in these pathways. For example, I can't keep up to all the caspases and intermediate steps. Of course many of these would be applicable to all types of cells outside of neurons. In relation to this, I am always looking for the best (reliable) method for showing apoptosis via immunocytochemistry in culture. There are so many kits. But is there one method that really stands out ie. works well, stains well etc.

Check with the company called: Cell Signaling Technology, Inc. Tel: 1-877-678-8324. They have great antibodies for Immunohistochemistry to study survival/apoptyosis and cell stress.

Hi,
Waht disorders do you work with.
I am interested in Autophagy neuronal cell death in AD.
Guy

parkinsonbu