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transformation

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cmdobson

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What is the best (most effective) method for transforming yeast with a PCR product for integration?

Lithium Acetate, Electrophorhesis, others?

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 Posted Nov 02, 2005, 15:26 PM
Galicola

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I used lithium acetate with no particular problems.
I never tried electrophoresis with a PCR product, but with a plasmid it gave a higher transformation efficiency.
you can find protocols here or there and other places (just google for yeast protocols.

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Posted Nov 07, 2005, 14:57 PM
cmdobson

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Thank-you for that information

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Posted Nov 07, 2005, 12:46 PM
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