What is the best (most effective) method for transforming yeast with a PCR product for integration?Lithium Acetate, Electrophorhesis, others?
I used lithium acetate with no particular problems.I never tried electrophoresis with a PCR product, but with a plasmid it gave a higher transformation efficiency.you can find protocols here or there and other places (just google for yeast protocols.
Thank-you for that information
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