Determination of copy number |
|
Would you like to save this topic, event, protocol or job so you can find it again easily?
Just click the "Save to My Lab Drawer" link and the item will be saved in the My Lab Drawer section of your bench space.
Available to members only. Please log in or register for your free account now.
|
|
Topic Started by deepti
on 10/4/2005 4:52 AM
|
|
How to determine copy number of a plasmid especially if isolating the plasmid from the organism remains a problem. I would also like to know if anyone has got a protocol for isolating plasmid from Deinococcus.
|
Replies
|
|
|
This article is a classic reference for copy number determination
Twigg, A. J., and D. Sherratt. 1980.
Trans-complementable copy-number mutants of plasmid ColEl. Nature (London) 283: 216-218.
|
|
|
|
This recent article describes the determination of plasmid copy number in bacteria using real time qPCR. The authors also compared their results with the agarose gel assay.
Lee, CL et al. Quantitative real-time polymerase chain reaction for determination of plasmid copy number in bacteria. Journal of Microbiological Methods, 65 (2006) 258-267.
|
|
As a Scientist Solutions member, you are able to register a positive vote for any topic which you believe is useful and relevant to our board or any reply which you believe is especially well worded and helpful.
By participating in the voting, you will be helping to identify the best topics & replies on the board.
You may vote once for any one post, and you may not vote for your own posts.
A post (topic or reply) will earn one "thumbs up" icon for every 10 votes received (up to 3 thumbs up), and the person who made the post will also earn two bonus points.
learn more about member points.
|
Become a member & join our
community (It's easy & free)
Already a member? Please log in
|
Life Technologies
|
|
Life Technologies
|
|
TubeWriter
|
|
7/2/2010
|
|
6/22/2010
|
|
4/5/2010
|
|
8/6/2010
|
|
2/22/2010
|
|
12/28/2009
|
|
|