ALS or Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative disease known as: Lou Gehrig's disease.
ALS has a panel of twelve biomarkers that are effective in identifying patients with ALS.
A Surface Enhanced Laser Desorption/Ionizationor (SELDI) developped by Cipfergen Biosystems, Fremont, CA is the best sytem to detect these biomarkers. Does anyone know a better technology for such application? This technology is based on protein chip technology.

Roshan said:

ALS or Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative disease known as: Lou Gehrig's disease. ALS has a panel of twelve biomarkers that are effective in identifying patients with ALS. A Surface Enhanced Laser Desorption/Ionizationor (SELDI) developped by Cipfergen Biosystems, Fremont, CA is the best sytem to detect these biomarkers. Does anyone know a better technology for such application? This technology is based on protein chip technology.

Check the following companies: Cepheid, Illumina, Roche and Affymetrix

If you want to campare individual protein elements between patient and normal samples by mass spec, a labelling kit such as iTRAQ can give you enhanced qualitative and quantitative data. Of course, unlike your protein chip system, you can only look at one pair at a time, but runs are fast.

samm said:

If you want to campare individual protein elements between patient and normal samples by mass spec, a labelling kit such as iTRAQ can give you enhanced qualitative and quantitative data. Of course, unlike your protein chip system, you can only look at one pair at a time, but runs are fast.

Thanks Samm, the iTRAQ Reagent Kit is the first set of amine-specific, stable isotope reagents that can label all peptides in up to four different biological samples. This kit is made by Applied Biosystems. have you ever used this kit reagents ? what are the pros and cons of using this product? I have to come up with a list of the best products in the market to measure biomarkers.

[quote=omid][quote=samm]If you want to campare individual protein elements between patient and normal samples by mass spec, a labelling kit such as iTRAQ can give you enhanced qualitative and quantitative data. Of course, unlike your protein chip system, you can only look at one pair at a time, but runs are fast.[/quote]

Thanks Samm, the iTRAQ Reagent Kit is the first set of amine-specific, stable isotope reagents that can label all peptides in up to four different biological samples. This kit is made by Applied Biosystems. have you ever used this kit reagents ? what are the pros and cons of using this product? I have to come up with a list of the best products in the market to measure biomarkers.

Information on ClinProt biomarker discovery, ID, and
validation systems and ICPL is at www.bdal.com Mike W

Thanks Samm, the iTRAQ Reagent Kit is the first set of amine-specific, stable isotope reagents that can label all peptides in up to four different biological samples. This kit is made by Applied Biosystems. have you ever used this kit reagents ? what are the pros and cons of using this product? I have to come up with a list of the best products in the market to measure biomarkers.

We were looking to detect a specific protein cleavage fragment in patient samples - hence the iTRAQ was evaluated. Howeve, we later found that this fragment was predominantly produced only in the patient samples, so a straight mass spec provided the info we needed.
I am aware of one lab that has used it for labelling and studying conc. urine samples for certain carcinoma markers.