Award
 » Home » Analytical Chemistry » Electrophoresis (IEF, SDS-Page, 2D-Gel, Capillary, Native) » CE eof marker at 280 nm
 
Solutions Search! The Customized Life Science Search Engine
Search Site
Search Suppliers
Search Internet
Search over 6000 life science websites specifically selected by our expert scientist moderators.

Other Topics
4/7/2007 03:41 PM
Protein PEGylation
4/6/2007 03:13 PM
looking for helpful tips ...
5/19/2006 01:38 PM
2D-PAGE
4/27/2006 03:53 AM
urea/glycerol gel elecrop ...
4/24/2006 11:47 PM
2D electrphoresis of GST ...
2/7/2006 03:42 AM
Determination of protein ...
1/23/2006 06:43 PM
2D-PAGE
5/27/2005 10:10 AM
@D-PAGE analysis, and nor ...
12/18/2004 12:39 AM
SDS-Page standard markers
Subscribet to topic
bottom of page RSS Feed Topic Feed
 CE eof marker at 280 nm [View Printable]
SueC.

Frog Egg

See
Similar
Scientists





Group: Member
Posts: 8
Joined: Jan 03, 2005







 Send a personal messsage to SueC. Reply with a quote from this post Go to the top of the page

Is there an eof (neutral) marker for CE that absorbs at 280 nm? Most markers absorb at lower wavelengths, but I'm trying to eliminate non-protein peaks by looking only at 280 nm, but still need an eof marker to get mobilities.
Thanks in advance for any suggestions.
.........................

 Posted Sep 21, 2005, 10:21 AM
Sandy

Frog Laureate

See
Similar
Scientists





Group: Member
Posts: 117
Joined: Nov 23, 2004







 Send a personal messsage to Sandy Reply with a quote from this post Go to the top of the page

SueC. said:
Is there an eof (neutral) marker for CE that absorbs at 280 nm? Most markers absorb at lower wavelengths, but I'm trying to eliminate non-protein peaks by looking only at 280 nm, but still need an eof marker to get mobilities.
Thanks in advance for any suggestions.


May be checking the link below might help, it has a lot of information regarding capillary Electrophorsis:
http://www3.interscience.wiley.com/cgi-bin/abstract/106588657/ABSTRACT
.........................

Posted Jan 23, 2006, 15:08 PM
gsovak

Frog Laureate

See
Similar
Scientists





Group: Member
Posts: 544
Joined: Jan 25, 2005







 Send a personal messsage to gsovak Reply with a quote from this post Go to the top of the page

I am not sure that I am understanding your question.
Did you try a specific primary Ab against the eof and then a secondary fluorescent antibody?


SueC. said:
Is there an eof (neutral) marker for CE that absorbs at 280 nm? Most markers absorb at lower wavelengths, but I'm trying to eliminate non-protein peaks by looking only at 280 nm, but still need an eof marker to get mobilities.
Thanks in advance for any suggestions.
.........................

Posted Jan 25, 2006, 14:29 PM
mrmoon

Frog Egg

See
Similar
Scientists





Group: Member
Posts: 5
Joined: May 12, 2006







 Send a personal messsage to mrmoon Reply with a quote from this post Go to the top of the page

Benzene, if soluble in your matrix?
.........................

Posted May 19, 2006, 13:04 PM
top of page

Forum Jump