FLOW cytometry is new to me. Could someone please describe what the attached result image is showing?
Thanks!
BY

The histogram plot that you have is a composite anaysis of a few hundred to a few thousand individual "events" or cells that have singly passed in front of the laser, with physical and fluorescence properties being measured by a series of photo-multiplier tubes (PMTs). In most cytometers, data is measured across a series of "channels" (usually 1024) across multiple parameters. These include physical properties such as forward scatter (indicative of cell size), side scatter (indicative of internal/organelle complexity within the cell), and multiple fluorescence channels (usually Fl1, 2, etc) depending upon emission wavelengths of the fluorophore(s) used. In a "single-parameter" histogram plot, one usually sees the fluorescence intensity (increasing along x-axis) v/s the total number of cells at any given intensity ("counts").

Forgot to mntion - the two plots in your histogram overlay possibly represent a control flourescence sample (lower fluorecence) (can be cells alone, secondary antibody alone, or a non-specific antibody/rgt) and an actual experimental sample. This gives direct visual inference that your experiment sample has an enhanced *specific* fluorescence over the control. These plots can be "gated" or "subtracted" to obtain statistical analyses.