2D PAGEdetails |
|
Would you like to save this topic, event, protocol or job so you can find it again easily?
Just click the "Save to My Lab Drawer" link and the item will be saved in the My Lab Drawer section of your bench space.
Available to members only. Please log in or register for your free account now.
|
|
Topic Started by Tegrion
on 11/3/2009 1:11 AM
|
|
Hi, dear colleagues!
Looking for your opinion!
During perform 2D we suspest acrylamide and cystein group interactions leading to artefact results. When we perform 1D the same sample we got a lot of strips, but in 2D we have just a lot of spots and spindles on one level...
Could you propose something? How can we prove or disprove that?
And what do you think, how we can improve quality of separation? We work with affinity extracted protein (or mix) from plasma.
Thanks in advance!!
|
Replies
|
|
|
Posted By Ripal
on 12/1/2009 23:07 PM
|
|
Hi, guy
Tell you the truth, I always counter your question. I think your 2d-gel elements should be fresh. And your electrophoresis buffer solution need to change!
|
|
As a Scientist Solutions member, you are able to register a positive vote for any topic which you believe is useful and relevant to our board or any reply which you believe is especially well worded and helpful.
By participating in the voting, you will be helping to identify the best topics & replies on the board.
You may vote once for any one post, and you may not vote for your own posts.
A post (topic or reply) will earn one "thumbs up" icon for every 10 votes received (up to 3 thumbs up), and the person who made the post will also earn two bonus points.
learn more about member points.
|
Become a member & join our
community (It's easy & free)
Already a member? Please log in
|
Life Technologies
|
|
Life Technologies
|
|
TubeWriter
|
|
7/2/2010
|
|
7/2/2010
|
|
9/29/2009
|
|
8/31/2010
|
|
7/20/2010
|
|
12/28/2009
|
|
|