2D PAGEdetails

Hi, dear colleagues!

Looking for your opinion!

During perform 2D we suspest acrylamide and cystein group interactions leading to artefact results. When we perform 1D the same sample we got a lot of strips, but in 2D we have just a lot of spots and spindles on one level...

Could you propose something? How can we prove or disprove that?

And what do you think, how we can improve quality of separation? We work with affinity extracted protein (or mix) from plasma.

Thanks in advance!!








 

Hi, guy Tell you the truth, I always counter your question. I think your 2d-gel elements should be fresh. And your electrophoresis buffer solution need to change!