all mice died after injecting 4% sodium thioglycollate?

I use 4% sodium thioglycollate to elicit peritoneal macrophage from mouse.
sodium thioglycollate was aged for 6 weeks.
i injected 1ml per mouse, but after 4hours, all the mice died.
did everybody have similar problem? Expect good answers, thanks so much!

Hi,
I have read that you had a problem of lethality when you injected thioglycolate in your mice. I have injected 2 ml of 4% of SODIUM THIOGLYCOLATE from Sigma (T0632) and in less than an hour mi mice have died. How did you resolve this problem? Could anybody help my??

Thanks!!

Hi,

If the mice died in less than an hour, is it possible that your intraperitoneal injection technique may be causing some damage to the liver or a blood vessel?  Are you able to successfully inject saline as a control? 

Additionally, how do you prepare your thioglycollate solution?

First I made a 8% solution in water and after autoclaving it I made I prepared a 4% solution in PBS.
Do you think that the problem is the solution?

Thanks

Hi, my problem already solved by changing new thiogllycollate solution.
I read many references and found that, the one that could stimulate peritoneal macrophage is not sodium thiogllycollate, but AGE (advanced glycation endproducts), and AGE is produced by sodium thioglycollate and agar. So I suggest you to change your reagent,  Thioglycollate Broth from Fluka can work well for me.
Good luck!

Thank you for your help!!
Could you give me the reference of the thioglycolate you use and say me how do you prepare it exactly?

How much solution do you inject to the mice?

Thanks

the cat no. is 70157, from Fluka, make 4% thioglycollate as the datasheet said, and inject 1ml to each 6-week-old mice.

The datasheet says to suspend 29 g of Alternative Thioglycollate Medium in 1000 ml of distilled water. I supposed that it is the 100% solution. How do you prepare the 4% solution? Do you make it in PBS1x or in distilled water?

Thanks 

4% means the content of sodium thioglycollate, I use distilled water.

So if I understand well, you make the solution suspending 232g of the Thioglycollate Broth, USP Alternative (#70157) in 100 ml of distilled water getting the 4% solution that you inject to the mice.

I am sorry for so many questions but I am afraid of doing something wrong and killing all my mice again.
Thanks