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Human papillomavirus type 16 exists in bacteria

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Topic Started by wwwkkk83
on 9/24/2009 19:54 PM   
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Human papillomavirus type 16 exists in bacteria isolated from cervical cancer biopsies.

Ma Z, Liu L, Zhang F, Yu M, Wang K, Luo J, Liu K, Chen B, Xu L.
J Int Med Res. 2009 Jul-Aug;37(4):1065-74

 This study investigated the association between infectious microbes and persistent infection with human papillomavirus type 16 (HPV-16) in cervical cancer. Bacterial strains (identified as Enterococcus, Staphylococcus, Bacillus and Corynebacterium, based on their partial 16S rDNA sequence) were HPV-16 positive from 12 out of 14 cervical cancer biopsies. Total DNA was isolated from the four bacterial strains, and HPV-16 genes and genome were detected using polymerase chain reaction (PCR) and Southern blotting. RNA transcripts for HPV-16 E6 and L1 genes were detected in total bacterial RNA samples using reverse transcription-PCR, and HPV-16 L1 protein expression was detected in bacterial cells by Western blotting and immunocolloidal gold electron microscopy. The presence of virus particles in bacterial cells was demonstrated by transmission electron microscopy. The results suggest that bacteria carrying HPV-16 could provide a potential explanation for how infectious microbes contribute to the progression from HPV-16 infection to cervical cancer.

Attached file: Human Papillomavirus Type 16 Exists in Bacteria Isolated from Cervical Cancer Biopsies.pdf (2 downloads, 237KB)


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Posted By wwwkkk83
on 9/24/2009 22:04 PM   
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 The study demonstrated that the HPV-16 genome exists in bacterial strains isolated from cervical cancer biopsies, that HPV-16 genes can be transcribed and translated in these bacterial strains, and that the HPV-16 gene product can form virus particles in these bacteria. This is the first report to show that HPV exists in bacteria isolated from human tissue biopsies. It only confirms the existence of HPV-16 in the specific bacterial strains isolated, however, so further studies should be carried out to explain this phenomenon.

Last edited Sep 26, 2009, 7:07 AM by wwwkkk83


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Posted By parvoman
on 9/25/2009 9:53 AM   
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This is really amazing. To show that a human pathogenic virus can infect, replicate its genome, express its viral proteins, and even package new particles (electron microscopy) inside bacteria and thus act not only as a secondary vector but also as a virus factory should have resulted in this paper being published in one of the top 3 journals. I'm wondering why it is not in one of the top journals. It's difficult to say without having read the paper but it might all hinge on the question as to whether the virus particles seen via electron microscopy are infectious. ie. It could be that some degree of self-assembly of L1 protein has occurred which results in a mixture of virus like particles being made but if replication and packaging have not also occurred at the same time then you don't get infectious virus. Definately worth a look though!

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