Human papillomavirus type 16 exists in bacteria isolated from cervical cancer biopsies.
Ma Z, Liu L, Zhang F, Yu M, Wang K, Luo J, Liu K, Chen B, Xu L.
J Int Med Res.
This study investigated the association between infectious microbes and persistent infection
with human papillomavirus type 16 (HPV-16) in cervical cancer
. Bacterial strains (identified as Enterococcus, Staphylococcus, Bacillus and Corynebacterium, based on their partial 16S rDNA sequence
) were HPV-16 positive from 12 out of 14 cervical cancer
biopsies. Total DNA
was isolated from the four bacterial strains, and HPV-16 genes
were detected using polymerase chain reaction
) and Southern blotting
transcripts for HPV-16 E6 and L1 genes
were detected in total bacterial RNA
samples using reverse transcription
, and HPV-16 L1 protein expression
was detected in bacterial cells
by Western blotting
and immunocolloidal gold electron microscopy
. The presence of virus particles in bacterial cells was demonstrated by transmission electron microscopy.
The results suggest that bacteria carrying HPV-16
could provide a potential explanation for how infectious microbes contribute to the progression from HPV-16 infection
to cervical cancer