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L-15 in a CO2 incubator

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tanyag
Australia

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Topic Started by tanyag
on 8/17/2009 15:38 PM   
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I have read that A-72 cells will develop CPE when infected with CPV when grown on L-15 media. However, all of our cell lines are kept in 5% CO2. How do I compensate for the CO2 so my cells won't die in the L-15 media. I have tried A-72 in EMEM but no CPE occured.
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heehawmcduff
United States

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Posted By heehawmcduff
on 8/17/2009 8:36 AM   
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I recently posted this question:

http://www.scientistsolutions.com/t12285-adding+hepes+to+dmem.html

and I'm wondering if a similar approach would work with your L-15 media?
tanyag
Australia

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Posted By tanyag
on 8/18/2009 9:46 AM   
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So maybe I should try losts more HEPES and much less bicarbonate? Would any other components help?
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