Hello! I'm new here... :) Does anyone know how much 3D structure data is available to date for Restriction Enzymes? Hope you guys could do me a favour. Thanks! :)

Hi

Go to

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=structure

Use this to search for the crystal structure of your enzyme of interest. The search "Restriction Endonuclease" brings up 75 hits.

You will need a program that can open and view PDB files. These include RasMol, or Deepview (freeware)

Deep view can be downloaded at

http://swissmodel.expasy.org/spdbv/text/download.htm


The link below is a search for "Restriction Endonuclease Crystal Structure" in PubMed. If it does not work just enter the search term in quote yourself on the pubmed page. Should get you 124 hits.

Link to results of Pubmed Search

Other useful links that may, or may not be included in the above Pubmed search


http://biotech.uah.edu/chenlq/publications/EcoRII_JMB_reprint.pdf
http://www.biomedcentral.com/1472-6807/5/2
http://www.jbc.org/cgi/content/abstract/280/7/5605
http://www.nature.com/emboj/journal/v17/n18/abs/7591239a.html
http://biotech.uah.edu/chenlq/publications/ecorii.pdf
http://nar.oxfordjournals.org/cgi/content/abstract/30/4/876
http://www.fhcrc.org/science/labs/stoddard/papers/galburt_nsb_nv2000.pdf
http://www.fhcrc.org/science/labs/stoddard/Conj544/post_translat_splicing/duan_etal_cell1997.pdf