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Cytokine infiltration upon electroporation with DNA

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may

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Has anyone worked with in vivo electroporation of skeletal muscle with DNA? We observe an infiltration of cytokines and macrophages when this is done. However, with no DNA controls (electroporation only) we do not see this phenomenom. Any guesses as to why?
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Posted Oct 14, 2004, 12:54 PM
David E

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How quickly are you observing this immune response?
DNA alone and in particular DNA originating from bacteria containing CpG stimulatory sequences or viral DNA can induce a strong immune response on its own.
Check out Gene Ther. 2004 Oct;11 Suppl 1:S10-7.
Immune responses to gene therapy vectors: influence on vector function and effector mechanisms.
Bessis N et al
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Posted Oct 15, 2004, 5:59 AM
venky04

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may said:
Has anyone worked with in vivo electroporation of skeletal muscle with DNA? We observe an infiltration of cytokines and macrophages when this is done. However, with no DNA controls (electroporation only) we do not see this phenomenom. Any guesses as to why?


ssDNA and dsDNA can bind to receptors of the innate immune system (Toll-like receptors, TLRs) which are expressed by a variety of cells. Signaling via TLRs often culminates in NFkB activation via JAK/STAT, MAPK or ERK signaling cascades- al of which cause cytokines to be released locally. Injection of DNA is considered foreign and the host immune system responds to danger signals (inflammatory response).
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Posted Jan 14, 2005, 17:37 PM
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