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jarvizu
Mexico

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Status: Frog Egg
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Topic Started by jarvizu
on 7/21/2009 17:15 PM   
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hello:

I´m trying to identify a DNA binding protein by supershift assays. Anyone know a protocol to carry out this assay?, and concentration of antibody should I use?

thanks for your help
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R Bishop
United States

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Posted By R Bishop
on 7/21/2009 18:18 PM   
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For supershift  you can add 0.5 to 1.0ug of antibody to get a final volume of 10-20uL to load the gel. T

here are a bunch of EMSA protocols listed in the protocols section. A good non-radioactive protocol is found here

Supershift Protocol

If you have access to Current Protocols this is a very descriptive methods paper

www.scientistsolutions.com/a5163-protocol-mobility+shift+dna_binding+assay+using+gel+electrophoresis.aspx

 

 

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