I´m trying to identify a DNA binding protein by supershift assays. Anyone know a protocol to carry out this assay?, and concentration of antibody should I use?
For supershift you can add 0.5 to 1.0ug of antibody to get a final volume of 10-20uL to load the gel. T
here are a bunch of EMSA protocols listed in the protocols section. A good non-radioactive protocol is found here
If you have access to Current Protocols this is a very descriptive methods paper
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